promoter 종류

1. hU6 The human U6 (hU6) promoter is widely used to express short hairpin RNAs (shRNAs) in mammalian cells. 요런 CRISPR guide RNA 만들때도 사용하나봄.

Polymerase Ⅲ promoter, (Human U6, Human H1, Mouse U6) Pol Ⅲ계 promoter는 short strand RNA의 발현 효율이 높으며 특히 U6, H1 등의 Type Ⅲ에 속하는 Pol Ⅲ계 promoter는 임의의 서열을 전사하는데 적합 하다. All three types of RNA polymerase promoters (types I, II, and III) are currently in use, although RNA polymerase III promoters are most frequently employed because they offer several advantages [1...